guinea pig polyclonal anti-map2  (Synaptic Systems)

Journal: iScience

Article Title: Chronic modulation of cAMP signaling elicits synaptic scaling irrespective of activity

doi: 10.1016/j.isci.2024.110176

Figure Lengend Snippet: Compound 21 selectively acts on DREADD-expressing neurons (A) Experimental design. Lentiviral constructs were transfected on DIV 4 with virus carrying hM4D(Gi) or Empty Vector, and experiments were performed between DIV 14–21. (B) Representative images of immunostaining for mCherry-DREADD and MAP2 staining. Representative scale bar, 20 μm. (C) Representative current-clamp traces of spontaneous firing before (left) and after (right) acute 1 μM CMPD21 perfusion on control (CTRL), empty vector (EV), and hM4D(Gi) neurons. (D) Comparison of the mean action potential frequency (Hz) following CMPD21 wash (two-way ANOVA with Sidak’s multiple comparisons test, CTRL vs. CTRL + CMPD21 p = 0.889; EV vs. EV + CMPD21 p = 0.150; Gi vs. Gi + CMPD21 p = 0.015; N = 2–3, n = 7–12 per group). (E) Comparison of mean resting membrane potential (mV) (two-way ANOVA with Sidak’s multiple comparisons test, CTRL vs. CTRL + CMPD21 p = 0.276; EV vs. EV + CMPD21 p = 0.053; Gi vs. Gi + CMPD21 p < 0.0001; N = 2–3, n = 7–12 per group). (F) Representative raw traces of AMPA-mediated mEPSCs before (left) and after (right) acute 1 μM CMPD21 perfusion. (G) Comparison of the mean mEPSC amplitude (pA) following CMPD21 wash (two-way ANOVA with Dunnett’s multiple comparisons test, CTRL baseline vs. CTRL + CMPD21 p = 0.697; EV baseline vs. EV + CMPD21 p = 0.990; Gi baseline vs. Gi + CMPD21 p = 0.043; N = 2–3, n = 5–6 per group). (H) Comparison of the mean mEPSC frequency (Hz) following CMPD21 wash (two-way ANOVA with Dunnett’s multiple comparisons test, CTRL baseline vs. CTRL + CMPD21 p = 0.728; EV baseline vs. EV + CMPD21 p = 0.888; Gi baseline vs. Gi + CMPD21 p = 0.913; N = 2–3, n = 5–6 per group). Data are represented as mean ± SEM. Significant symbols used in figures are defined as such: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; n.s.

Article Snippet: Guinea pig polyclonal anti-MAP2 , Synaptic Systems , Cat#188004.

Techniques: Expressing, Construct, Transfection, Virus, Plasmid Preparation, Immunostaining, Staining, Control, Comparison, Membrane

Journal: iScience

Article Title: Chronic modulation of cAMP signaling elicits synaptic scaling irrespective of activity

doi: 10.1016/j.isci.2024.110176

Figure Lengend Snippet:

Article Snippet: Guinea pig polyclonal anti-MAP2 , Synaptic Systems , Cat#188004.

Techniques: Recombinant, Plasmid Preparation, Membrane, Transfection, Generated, Software

Journal: STAR Protocols

Article Title: Super-resolution imaging of synaptic scaffold proteins in rat hippocampal neurons

doi: 10.1016/j.xpro.2023.102080

Figure Lengend Snippet: Antibody concentrations and dilutions

Article Snippet: Anti-MAP2 (guinea pig polyclonal) , Synaptic Systems , Cat# 188-004 RRID: AB_2138181.

Techniques: Concentration Assay

Journal: STAR Protocols

Article Title: Super-resolution imaging of synaptic scaffold proteins in rat hippocampal neurons

doi: 10.1016/j.xpro.2023.102080

Figure Lengend Snippet:

Article Snippet: Anti-MAP2 (guinea pig polyclonal) , Synaptic Systems , Cat# 188-004 RRID: AB_2138181.

Techniques: Recombinant, Plasmid Preparation, Software

Journal: bioRxiv

Article Title: Synaptojanin1 regulates lysosomal functions in ventral midbrain neurons

doi: 10.1101/2022.10.14.512269

Figure Lengend Snippet: Reduced LAMP1 expression and puncta number in Synj1 deficient MB neurons. (A)-(B) Immunofluorescence analysis of LAMP1 in the soma of Synj1+/+ and Synj1+/- MB neurons, MAP2 is used as neuronal marker. (A), representative images. (B), quantification results, N=82/88 ( Synj1+/+ / Synj1+/- ) neurons. (C) Quantification of LAMP1 labeled puncta for Synj1+/+ and Synj1+/- MB neurons. Y axis represents the normalized puncta number per unit cytoplasmic area, N=82/88 ( Synj1+/+ / Synj1+/- ) neurons. (D) Cumulative frequency graph shows the LAMP1 fluorescence intensity distribution of the puncta in soma between Synj1+/+ and Synj1+/- MB neuron. N=2091/2873 ( Synj1+/+ / Synj1+/- ) puncta. (E)-(F) Analysis of LAMP1-GFP puncta in the axons of Synj1+/+ and Synj1+/- MB neurons. (E), representative images. (F), quantification results, N=25/31 ( Synj1+/+ / Synj1+/- ) axons. The p values for (B), (C) and (F) are from student’s t -test. The p value for (D) is from Two Sample Kolmogorov-Smirnov test. Scale bar in all images, 10 μM.

Article Snippet: The anti-MAP2 Polyclonal Chicken Antibody (188 006, 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Expressing, Immunofluorescence, Marker, Labeling, Fluorescence

Journal: bioRxiv

Article Title: Synaptojanin1 regulates lysosomal functions in ventral midbrain neurons

doi: 10.1101/2022.10.14.512269

Figure Lengend Snippet: Reduced LAMP2 and LAMP2A in Synj1 deficient MB neurons. (A)-(B) Immunofluorescence analysis of LAMP2 in the soma of Synj1+/+ and Synj1+/- MB neurons, MAP2 is used as neuron marker. (A), representative images. (B), quantification results. (C)-(D) Immunofluorescence analysis of LAMP2A in the soma of Synj1+/+ and Synj1+/- MB neurons, MAP2 is used as neuron marker. (C), representative images. (D), quantification results. The p values for (B) and (D) are from student’s t -test. Scale bar in all images, 10 μM.

Article Snippet: The anti-MAP2 Polyclonal Chicken Antibody (188 006, 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Immunofluorescence, Marker

Journal: bioRxiv

Article Title: Synaptojanin1 regulates lysosomal functions in ventral midbrain neurons

doi: 10.1101/2022.10.14.512269

Figure Lengend Snippet: Synj1 deficiency leads to lysosomal hyperacidification and enhanced enzymatic activity. (A)-(B) Lysosome acidity assay using a ratiometric fluorescent probe pHLy. (A), representative images show the mCherry signal and mTFP1 signal in the soma of MB neuron transfected with pHLy. (B), distribution analysis of the ratio (mCherry/mTFP1) for the pHLy labeled puncta between Synj1+/+ and Synj1+/- MB neurons. N=1151/1108 ( Synj1+/+ / Synj1+/- ) puncta. (C)-(D) Lysosome acidity assay in the axon of MB neuron. (C), representative images show the mCherry signal and mTFP1 signal in axon. (D), distribution analysis of the ratio (mCherry/mTFP1) for the pHLy labeled puncta between Synj1+/+ and Synj1+/- axons. N=432/579 ( Synj1+/+ / Synj1+/- ) puncta. (E)-(F) Cathepsin-B activity analysis using the dye Magic Red. (E), representative images show the Magic Red signal from live cell imaging and the MAP2 signal from post hoc staining for Synj1+/+ and Synj1+/- MB neurons. (F), quantification of the intensity of Magic Red signal between Synj1+/+ and Synj1+/- MB neurons. N=78/159 ( Synj1+/+ / Synj1+/- ) neurons. The P values for (B) and (D) are from Two Sample Kolmogorov-Smirnov test. The p value for (F) is from student’s t -test. Scale bar in (A) and (C), 5 μM; Scale bar in (E), 10 μM.

Article Snippet: The anti-MAP2 Polyclonal Chicken Antibody (188 006, 1:1000 for IF) and the anti-MAP2 Polyclonal Guinea Pig Antibody (188 004, 1:1000 for IF) were purchased from Synaptic Systems (Goettingen, Germany).

Techniques: Activity Assay, Transfection, Labeling, Live Cell Imaging, Staining